| Acronym | Meaning |
 | LRO | Large Radio Observatory |
| LRR | Launch Readiness Review |
| LRSI | Low Temperature Reusable Surface Insulation |
| LRSI | Low-temperature Reusable Surface Insulation |
| LRU | Line Replaceable Unit |
| LRU | Line-replaceable Unit |
| LRV | Launch Readiness Verification |
| LRV | Long Range Video |
| LS | Landing Side |
| LS | Launch Sequence |
| LS | Left Side |
| LS | Life Science |
| LS | Limit Switch |
| LS/ST | Light Shield/Star Tracker |
| LSA | Launch Services Agreement |
| LSA | Logistics Support Analysis |
| LSAH | Launch Site Accommodations Handbook |
| LSAP | Launch Sequence Applications Program |
| LSB | Least Significant Bit |
| LSB | Lower Side Band |
| LSC | Linear Shaped Charge |
| LSCA | Logistics Support Cost Analysis |
| LSD | Landing Ship Dock |
| LSD | Low Speed Data |
| LSE | Launch Support Equipment |
| LSE | Life Support Equipment |
| LSF | Laboratory Simulation Facility |
| LSFE | Life Sciences Flight Experiment |
| LSI | Large Scale Integration |
| LSID | Launch Sequence And Interlock Document |
| LSM | Life Science Module |
| LSM | ILogistics Support Management Information |
| LSO | Landing Support Officer |
| LSO | Large Solar Observatory |
| LSP | Least Significant Portion |
| LSPDF | Life Science Payloads Development Facility |
| LSR | Land Sea Rescue |
| LSR | Launch Site Recovery |
| LSRM | Life Science Research Module |
| LSS | Landing |
| LSS | Life Support Subsystem |
| LSS | LLife Sciences Space Laboratory |
| LSSF | Life Sciences Support Facility |
| LSSM | Launch Site Support Manager |
| LSSP | Launch Site Support Plan |
| LSSRC | Life Sciences Shuttle Research Centrifuge |
| LSST | Launch Site Support Team |
| LST | Large SpaceTelescope |
| LST | Large Stellar Telescope |
| LST | LaunchSupportTeam |
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